Destrin Polyclonal Antibody, Cy5.5 Conjugated
Product Specifications
Background
Actin-depolymerizing factor (ADF), also known as destrin, is a member of the ADF/Cofilin/destrin superfamily that has the ability to rapidly depolymerize F-Actin in a stoichiometric manner. The Actin-depolymerizing activity of ADF is reversibly controlled by changes in KCl concentration but is insensitive to calcium concentration. ADF depolymerizes F-Actin by interacting directly with F-Actin protomers. ADF shares 71% sequence homology with Cofilin, however the two proteins differ in their interaction with Actin. The difference in the function of ADF and Cofilin results from the subtle difference in their amino acid sequence rather than possible differences in posttranslational modifications. As a result of different cleavage sites on ADF and Cofilin, the proteins differ in their overall tertiary folds. Sensitivity to polyphosphoinositides may be a common feature in vitro among Actin-binding proteins such as ADF and Cofilin that can bind to G-Actin and regulate the state of Actin polymerization. ADF and Cofilin are Actin-depolymerizing proteins whose activities are possibly regulated by their phosphorylation/dephosphorylation.
Synonyms
2610043P17Rik; ACTDP; Actin depolymerizing factor; Actin-depolymerizing factor; ADF; AU042046; bA462D18.2; corn1; DEST_HUMAN; Destrin actin depolymerizing factor; Destrin; DSN; Dstn; Sid 23; sid23p.
Gene ID
11034
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Immunogen
75-165/165
Target
Destrin
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy5.5
Source
KLH conjugated synthetic peptide derived from human Destrin
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
675nm/694nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.biossantibodies.com/datasheets/bs-12997R-Cy5.5
Gene ID URL
11034
Predicted Cross Reactivity
Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Monkey
Curated Selection
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