Phospho-CK18 (Ser52) Polyclonal Antibody, RBITC Conjugated

Product Specifications

Background

When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity) . Together with KRT8, is involved in interleukin-6 (IL-6) -mediated barrier protection.

Synonyms

Keratin, type I cytoskeletal 18; Cytokeratin endo B; Keratin D; Cytokeratin-18; CK-18; Keratin-18; K18; Krt18; Kerd; Krt1-18

Gene ID

16668

Modification Site

Ser52

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Mouse, Rat

Target

Phospho-CK18 (Ser52)

Clonality

Polyclonal

Isotype

IgG

Conjugation

RBITC

Source

KLH conjugated synthetic phosphopeptide derived from mouse CK18 around the phosphorylation site of Ser52 [SR (p-S) VW]

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

570nm/595nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

16668

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