Argonaute 3 Polyclonal Antibody, APC Conjugated
Product Specifications
Background
Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA) -containing ribonucleoprotein particle (miRNP) . Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5) .
Synonyms
Argonaute 3/e2C 3; 5730550L01Rik; Ago 3; Ago3; AGO3_HUMAN; argonaute 3; Argonaute3; e 2C 3; e-2C 3; e2C 3; E2c3; e2C3; E2C3 protein; Eukaryotic translation initiation factor 2C 3; Eukaryotic translation initiation factor 2C3; FLJ12765; hAgo3; MGC86946; Protein argonaute-3.
Gene ID
192669
Cellular Locus
Cytoplasm
Host
Rabbit
Immunogen
251-350/860
Target
Argonaute 3
Clonality
Polyclonal
Isotype
IgG
Conjugation
APC
Source
KLH conjugated synthetic peptide derived from human Argonaute 3/eIF2C3
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
650nm/660nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
192669
Predicted Cross Reactivity
Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, Zebrafish
Curated Selection
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