Phospho-APC (Ser2054) Polyclonal Antibody, Cy3 Conjugated

Product Specifications

Background

Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF) -induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

Synonyms

APC phospho S2054; p-APC phospho S2054; Adenomatous Polyposis Coli; APC; CC1; DP2; DP2.5; DP3; FAP; FPC; GS; Protein APC.

Gene ID

324

Modification Site

Ser2054

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Mouse, Rat

Target

Phospho-APC (Ser2054)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy3

Source

KLH conjugated synthetic phosphopeptide derived from human APC around the phosphorylation site of Ser2054

Applications

IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

512,550nm/570,615nm

Concentration

1µg/µl

Dilution

IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

324

Predicted Cross Reactivity

Human, Dog, Cow, Sheep, Pig, Rabbit

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

BM571 50 µL

Laminin alpha 5 (LAMA5) Mouse Monoclonal Antibody [Clone ID: 2D8/33 (4C7)]

Ask

View Details

ANT-35858-100ug 100 µg

Human ERG Antibody

Ask

View Details

NBP2-27110SS 0.025 mg

Rabbit Polyclonal HES2 Antibody

Ask

View Details

NBP2-79437-15Plates 15 Plates

Mouse Monoclonal PD-L1 Antibody Pair [HRP]

Ask

View Details

E56G3993 100 µL

RAC3 Rabbit Monoclonal Antibody

Ask

View Details

E-AB-F1117M-01 20 Tests

Elab Fluor® 647 Anti-Human CD123 Antibody[6H6]

Ask

View Details