AGO2 Polyclonal Antibody
Product Specifications
Background
Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA) -containing ribonucleoprotein particle (miRNP) . Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5) .
Synonyms
Ago 2; AGO2_HUMAN; Argonaute 2; Argonaute2; dAgo2; e 2C 2; e-2C 2; e2C 2; E2c2; Eukaryotic translation initiation factor 2C 2; hAgo2; MGC3183; PAZ Piwi domain protein; PPD; Protein argonaute-2; Protein slicer; Q10; Slicer protein.
Gene ID
27161
Swiss Prot
Q9UKV8
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Mouse
Immunogen
431-530/859
Target
AGO2
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human eIF2C2/Ago2
Applications
WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
27161
Predicted Cross Reactivity
Human, Rat, Dog, Cow, Sheep, Pig, Horse
More Discoveries
Explore Other Products
Browse additional items from our catalog
MBS6504260-01 0.1 mL
TYRO3 (Tyrosine-protein Kinase SKY, BYK, DTK, RSE, Tyrosine-protein Kinase Receptor TYRO3, Tyrosine-protein Kinase RSE, Tyrosine-protein Kinase SKY, Tyrosine-protein Kinase DTK, Tyrosine-protein Kinase byk) (MaxLight 490)
Sign In for Pricing
View Details MBS6504260-02 5x 0.1 mL
TYRO3 (Tyrosine-protein Kinase SKY, BYK, DTK, RSE, Tyrosine-protein Kinase Receptor TYRO3, Tyrosine-protein Kinase RSE, Tyrosine-protein Kinase SKY, Tyrosine-protein Kinase DTK, Tyrosine-protein Kinase byk) (MaxLight 490)
Sign In for Pricing
View Details