X-Gluc (Dicyclohexylamine)

X-Gluc Dicyclohexylamine is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system) [1].

Product Specifications

CAS Number

[18656-96-7]

UNSPSC

12171500

Target

Fluorescent Dye; β-glucuronidase

Type

Dye Reagents

Related Pathways

Metabolic Enzyme/Protease; Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/X-Gluc-Dicyclohexylamine.html

Concentration

10mM

Purity

99.48

Solubility

DMSO : ≥ 30 mg/mL

Smiles

O[C@H]([C@@H](O)[C@@H]1O)[C@@H](O[C@@H]1C(O)=O)OC2=CNC3=C2C(Cl)=C(Br)C=C3.C4(CCCCC4)NC5CCCCC5

Molecular Formula

C26H36BrClN2O7

Molecular Weight

603.93

References & Citations

[1]Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51 (5) :402-404.