SHC2 Polyclonal Antibody
Product Specifications
Background
Src homology 2 (SH2) domains bind specifically to tyrosine-phosphorylated proteins that temporally participate in signal transduction events (1) . Shc-like protein (Sck) is a neuronal adaptor protein that contains an N-terminal PTB (phosphotyrosine binding) domain, a collagen homology (CH) domain, and a conserved C-terminal SH2 domain (2,3) . Human Sck transcripts are present at high levels in liver, pancreas, prostate and ovary (4,5) . In vascular endothelial cells, Sck participates in VEGF-induced signal transduction (6) . Treatment of human umbilical vein endothelial (HUVEC) cells with VEGF induces recruitment of Sck to tyrosine-1175 of the kinase insert domain-containing receptor (KDR) and enhances Sck tyrosine phosphorylation (7,8) .
Synonyms
Protein Sck; SCK; SH2 domain protein C2; SHC transforming protein 2; SHCB; Src homology 2 domain containing transforming protein C2; SHC2_HUMAN.
Cellular Locus
Cytoplasm, Cell membrane
Host
Rabbit
Immunogen
161-270/582
Target
SHC2
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human SHC2/Sck
Applications
ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Predicted Cross Reactivity
Human, Mouse, Rat, Dog, Cow, Sheep
Curated Selection
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