ATBF1 Polyclonal Antibody, HRP Conjugated
Product Specifications
Background
AT-motif binding factor 1 (ATBF1) binds to the AT-rich core sequence element in the human a-fetoprotein enhancer (1) . Alternative splicing generates the ATBF1-A and ATBF1-B (2,3) . While ATBF1-A contains a 920-amino acid extension at the N-terminus, both ATBF1-A and ATBF1-B contain 4 DNA-binding homeobox domains (2,3) . Additionally, ATBF1-A contains 23 zinc finger motifs while ATBF1-B contains 18 zinc finger motifs (13) . The N-terminal extension unique to ATBF1-A has transcriptional repressor activity (4) . In the small intestine, ATBF1-A inhibits expression of the brushborder enzyme aminopeptidase-N through direct binding to the AT motif element (5) . Besides functioning in transcription regulation, ATBF1 also functions in ATPase activity (6) . ATPase activity associated with ATBF1-A is DNA/RNA-dependent and requires both homeobox domains and zinc finger motifs (6) . ATBF1 is highly expressed in spleen and brain tissues (7) . The gene encoding human ATBF1 maps to chromosome 16q22.3-q23.1 (8) .
Synonyms
Alpha fetoprotein enhancer binding protein; AT binding transcription factor 1; AT mot binding factor; ATBT; ZFHX3; ZFHX3_HUMAN.
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Rat
Immunogen
301-400/3703
Target
ATBF1
Clonality
Polyclonal
Isotype
IgG
Conjugation
HRP
Source
KLH conjugated synthetic peptide derived from human ATBF1
Applications
ELISA, IHC-P, IHC-F
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Predicted Cross Reactivity
Human, Mouse, Dog, Cow, Sheep, Horse, Chicken, Rabbit
Curated Selection
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