CHEK2 Polyclonal Antibody, APC Conjugated
Product Specifications
Background
DNA damage results in the arrest of cell cycle progression, allowing the damaged DNA to be repaired prior to replication. Checkpoints exist at several cell cycle phase transitions to maintain this genetic integrity. Rad9, Rad17, Rad24 and Mec3 are involved in activating the G1 and G2 checkpoints (14) . Pol2 (also known as Dun2), encoding the catalytic subunit of DNA polymerase epsilon, plays a role in activating the S phase checkpoint (5) . The protein kinase Rad53 (also designated Spk1, Mec2 or Sad1) is essential for both G2 and S phase arrest. Activation of Rad53 is regulated by Mec1 (also known as Esr1 and Sad3), a homolog of the human ATM protein (6) . Pds1 and Mad2 both regulate checkpoints associated with incomplete spindle replication (7,8) . Dun1, another protein kinase, plays a role in transducing the DNA damage signal (9) .
Synonyms
CHEK2 homolog; CHK2 homolog; MEC2; RAD53; RAD53_YEAST; Serine protein kinase 1; Serine-protein kinase 1; Serine/threonine-protein kinase RAD53; SPK1.
Cellular Locus
Cell membrane
Host
Rabbit
Cross Reactivity
Human, Mouse
Immunogen
101-200/543
Target
CHEK2
Clonality
Polyclonal
Isotype
IgG
Conjugation
APC
Source
KLH conjugated synthetic peptide derived from human Rad53/SPK1
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
650nm/660nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Predicted Cross Reactivity
Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
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