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NHLH2 Polyclonal Antibody, Cy3 Conjugated

Product Specifications

Background

The helix-loop-helix (HLH) structures are known motifs commonly found in membrane-active and DNA-binding proteins. The helix-loop-helix proteins HEN1 and HEN2 are DNA-binding proteins that may be involved in cell-type determination in the early nervous system. Studies of expression in normal tissues have demonstrated expression of NHLH1/NSCL-1 and NHLH2/NSCL-2, the genes encoding HEN1 and HEN2, in the developing central and peripheral nervous system, specifically in developing neurons.

Synonyms

HEN2; Nescient helix loop helix 2; NSCL; NSCL2; HEN2_HUMAN.

Cellular Locus

Nucleus

Host

Rabbit

Cross Reactivity

Human

Immunogen

1-50/135

Target

NHLH2

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy3

Source

KLH conjugated synthetic peptide derived from human NHLH2

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

512,550nm/570,615nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Predicted Cross Reactivity

Mouse, Rat, Dog, Cow, Pig

Frequently Asked Questions

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