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Oct-3/Oct-4 Polyclonal Antibody, AbBy Fluor™ 555 Conjugated

Product Specifications

Background

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') . Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Synonyms

Oct3; Oct4; Otf3; Otf4; NF-A3; Oct-3; Oct-4; Otf-3; Otf-4; OCT 4; Otf3g; Oct3/4; Oct-3/4; Otf3-rs7; POU domain, class 5, transcription factor 1; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; Pou5f1

Gene ID

18999

Swiss Prot

P20263

Cellular Locus

Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Immunogen

201-300/360

Target

Oct-3/Oct-4

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 555

Source

KLH conjugated synthetic peptide derived from mouse OCT-4

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

553nm/568nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

18999

Predicted Cross Reactivity

Dog, Cow, Pig

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