MID2 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Product Specifications
Background
Midline-2 is a 715 amino acid protein encoded by the human gene MID2. Midline-2 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, one B30.2/SPRY domain, one COS domain, one fibronectin type-III domain and one RING-type zinc finger. Midline-2 is a cytoplasmic protein found as a homodimer or heterodimer with Midline-1. It also interacts with IGBP1 (Lymphocyte signaling protein A4) . Dimerization is mediated by the tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) and microtubule association is dependent on the C-terminal B30.2 domain. Midline-2 is expressed at low levels in fetal kidney and lung, and in adult prostate, ovary and small intestine.
Synonyms
FXY2; MID2; Midin-2; Midline 2; Midline defect 2; Midline-2; Probable E3 ubiquitin-protein ligase MID2; RING finger protein 60; Ring finger protein protein 60; RNF60; TRIM1; TRIM1_HUMAN; Tripartite mot protein 1; Tripartite mot-containing protein 1; Tripartite mot-containing protein 1; Midline defect 2; FLJ37715; FLJ41813.
Gene ID
26040
Host
Rabbit
Immunogen
251-350/735
Target
MID2
Clonality
Polyclonal
Isotype
IgG
Conjugation
PerCP-Cy5.5
Source
KLH conjugated synthetic peptide derived from human RNF60/Midline-2
Applications
IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
482nm/694nm
Concentration
1µg/µl
Dilution
IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
26040
Predicted Cross Reactivity
Human, Mouse, Rat, Dog, Sheep, Pig, Horse, Chicken
Curated Selection
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