His Tag Polyclonal Antibody, Biotin Conjugated

Product Specifications

Background

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Synonyms

HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag.

Host

Rabbit

Cross Reactivity

Tags, Others

Target

His Tag

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Source

KLH conjugated synthetic peptide derived from His Tag

Applications

WB, ELISA, IHC-P, IHC-F

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C for 12 months.

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