Phospho-NLK (Thr298) Polyclonal Antibody, RBITC Conjugated
Product Specifications
Background
Nemo-like kinase (NLK) plays a role in cell fate determination and is required for differentiation of bone marrow stromal cells. It acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. It may suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP. (referenced from swissprot)
Synonyms
Nemo-like kinase Thr298; Nemo-like kinase T298; p-Nemo-like kinase Thr298; p-Nemo-like kinase T298; DKFZp761G1211; FLJ21033; LAK1; Nemo like kinase; Nemo-like kinase; Nlk; NLK_HUMAN; Protein LAK1; Serine/threonine protein kinase NLK; Serine/threonine-protein kinase NLK.
Modification Site
Thr298
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse
Target
Phospho-NLK (Thr298)
Clonality
Polyclonal
Isotype
IgG
Conjugation
RBITC
Source
KLH conjugated synthetic phosphopeptide derived from human Nemo-like kinase around the phosphorylation site of Thr298
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
570nm/595nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Predicted Cross Reactivity
Rat, Cow, Sheep, Pig, Chicken, Rabbit
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
MR207136L4V 200 µL
Slc18a2 (BC078449) Mouse Tagged ORF Clone Lentiviral Particle
Sign In for Pricing
View Details 
bsm-61060R-BF647-TR 20 µL
PIK3R3 Recombinant Antibody, AbBy Fluor™ 647 Conjugated
Sign In for Pricing
View Details bs-6631R-BF488 100 µL
Neurotensin Polyclonal Antibody, AbBy Fluor™ 488 Conjugated
Sign In for Pricing
View Details