Phospho-NLK (Thr298) Polyclonal Antibody
Product Specifications
Background
Nemo-like kinase (NLK) plays a role in cell fate determination and is required for differentiation of bone marrow stromal cells. It acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. It may suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP. (referenced from swissprot)
Synonyms
Nemo-like kinase Thr298; Nemo-like kinase T298; p-Nemo-like kinase Thr298; p-Nemo-like kinase T298; DKFZp761G1211; FLJ21033; LAK1; Nemo like kinase; Nemo-like kinase; Nlk; NLK_HUMAN; Protein LAK1; Serine/threonine protein kinase NLK; Serine/threonine-protein kinase NLK.
Modification Site
Thr298
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse
Target
Phospho-NLK (Thr298)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic phosphopeptide derived from human Nemo-like kinase around the phosphorylation site of Thr298
Applications
WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Predicted Cross Reactivity
Rat, Cow, Sheep, Pig, Chicken, Rabbit
Available Sizes
Curated Selection
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