Phospho-AKT1 (Tyr474) Polyclonal Antibody, Biotin Conjugated

Product Specifications

Background

AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Synonyms

RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; RAC

Gene ID

207

Modification Site

Tyr474

Cellular Locus

Cytoplasm, Nucleus, Cell membrane

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

Phospho-AKT1 (Tyr474)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Source

KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Tyr474

Applications

WB, ELISA, IHC-P, IHC-F

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C for 12 months.

Specificity

This antibody detects Akt1 when phosphorylated at Tyr474 and correspondingly, Akt2 when phosphorylated at Tyr 475 and Akt3 when phosphorylated at Tyr473