Histone H1.4 (Acetyl K53) Polyclonal Antibody, RBITC Conjugated

Product Specifications

Background

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity) .

Synonyms

H1E; H1.4; H1F4; H1s-4; dJ221C16.5; Histone H1.4; Histone H1b; Histone H1s-4; HIST1H1E

Gene ID

3008

Swiss Prot

P10412

Modification Site

Lys52

Cellular Locus

Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Histone H1.4 (Acetyl K53)

Clonality

Polyclonal

Isotype

IgG

Conjugation

RBITC

Source

KLH conjugated synthetic acetylpeptide derived from human Histone H1.4 around acetylation site of Lys52

Applications

FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

570nm/595nm

Concentration

1µg/µl

Dilution

FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Acetylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Specificity

This antibody will react with members of the Histone H1 family that have an acetylated lysine positioned in the motif AS (Ac-K) ER. This include H1.1, H1.2, H1.3, H1.4, and H1.5.