Histone H1.4 (Acetyl K53) Polyclonal Antibody
Product Specifications
Background
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity) .
Synonyms
H1E; H1.4; H1F4; H1s-4; dJ221C16.5; Histone H1.4; Histone H1b; Histone H1s-4; HIST1H1E
Gene ID
3008
Swiss Prot
P10412
Modification Site
Lys52
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Histone H1.4 (Acetyl K53)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic acetylpeptide derived from human Histone H1.4 around acetylation site of Lys52
Applications
ELISA, FCM, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
ELISA (1:500-1000), FCM (1:20-100), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Acetylation
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Specificity
This antibody will react with members of the Histone H1 family that have an acetylated lysine positioned in the motif AS (Ac-K) ER. This include H1.1, H1.2, H1.3, H1.4, and H1.5.
Gene ID URL
3008
Predicted Cross Reactivity
Dog, Cow, Pig, Horse
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