Phospho-BAD (Ser128) Polyclonal Antibody, AbBy Fluor™ 405 Conjugated
Product Specifications
Background
Promotes cell death. Successfully competes for the binding to Bcl-X (L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X (L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Synonyms
Bbc2; AI325008; Bcl2-associated agonist of cell death; BAD; Bcl-2-binding component 6; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; Bbc6
Gene ID
12015
Swiss Prot
Q61337
Modification Site
Ser128
Cellular Locus
Cytoplasm, Cell membrane
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-BAD (Ser128)
Clonality
Polyclonal
Isotype
IgG
Conjugation
AbBy Fluor™ 405
Source
KLH conjugated synthetic phosphopeptide derived from mouse BAD around the phosphorylation site of Ser128
Applications
WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
401nm/421nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Specificity
This phophorylation site is homologous to that of Ser129 in Rat.
Gene ID URL
12015
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