Phospho-AKT (Ser473) Polyclonal Antibody, HRP Conjugated
Product Specifications
Background
AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
Synonyms
Akt; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha; Akt1
Gene ID
24185
Swiss Prot
P47196
Modification Site
Ser473
Cellular Locus
Cytoplasm, Nucleus, Cell membrane
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-AKT (Ser473)
Clonality
Polyclonal
Isotype
IgG
Conjugation
HRP
Source
KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473
Applications
WB, IHC-P, IHC-F
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:500-2000), IHC-P (1:100-500), IHC-F (1:100-500)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
24185
Available Sizes
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