Phospho-AKT (Ser473) Polyclonal Antibody, APC-Cy5.5 Conjugated
Product Specifications
Background
AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
Synonyms
Akt; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha; Akt1
Gene ID
24185
Swiss Prot
P47196
Modification Site
Ser473
Cellular Locus
Cytoplasm, Nucleus, Cell membrane
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-AKT (Ser473)
Clonality
Polyclonal
Isotype
IgG
Conjugation
APC-Cy5.5
Source
KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473
Applications
WB, IF
Purification
Purified by Protein A.
Excitation Emission
650nm/694nm
Concentration
1µg/µl
Dilution
WB (1:500-2000), IF (1:100-500)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
24185
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC040806-500 1x 500 µL
TYRP1 (Tyrosinase Related Protein 1) (TA99), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC400994-100 1x 100 µL
TNF alpha (J2D10), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC471815-100 1x 100 µL
Chromogranin A / CHGA (Neuroendocrine Marker) (CHGA/1815R), CF647 conjugate, 0.1mg/mL
Sign In for Pricing
View Details