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Tyrosinase Polyclonal Antibody, PerCP-Cy5.5 Conjugated

Product Specifications

Background

Tyrosinase is the key enzyme for melanin, synthesis in mammalian melanocytes and has been considered to be a unique marker for the study of melanocyte differentiation. A cDNA library was constructed from poly (A) + mRNA from mouse melanocytes and screened using anti- tyrosinase antiserum and oligonucleotide probes corresponding to amino acid sequence of tyrosinase. sequencing of some cDNA clones positive in these screenings gave a nucleotide sequence of 1838 nucleotides including a open reading frame of 1344 nucleotides that was found to correspond exactly to the amino acid sequence of the cyanogen bromide fragments of tyrosinase.

Synonyms

LB24 AB; LB24-AB; Monophenol monooxygenase; SK29 AB; Tumor rejection antigen AB; LB24 AB; Monophenol monooxygenase; OCA1A; OCAIA; Oculocutaneous albinism IA; SK29 AB; SK29-AB; Tumor rejection antigen AB; TYR; tyrosinase oculocutaneous albinism IA; TYRO_HUMAN.

Gene ID

7299

Cellular Locus

Cytoplasm

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Immunogen

155-250/529

Target

Tyrosinase

Clonality

Polyclonal

Isotype

IgG

Conjugation

PerCP-Cy5.5

Source

KLH conjugated synthetic peptide derived from human Tyrosinase

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

482nm/694nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

7299

Predicted Cross Reactivity

Dog, Cow, Sheep, Pig

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