Collagen IV Polyclonal Antibody

Product Specifications

Background

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin (By similarity) .

Synonyms

Bru; Raw; Svc; Col4a-1; Del (8) 44H; Collagen alpha-1 (IV) chain; Col4a1

Gene ID

12826

Swiss Prot

P02463

Cellular Locus

Secreted, Cell membrane, Extracellular matrix

Host

Rabbit

Cross Reactivity

Human

Immunogen

1401-1500/1669

Target

Collagen IV

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

KLH conjugated synthetic peptide derived from mouse Collagen alpha-1 (IV) chain

Applications

WB, ELISA, FCM, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

WB (1:300-5000), ELISA (1:500-1000), FCM (1:20-100), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)