Vimentin Polyclonal Antibody, APC-Cy7 Conjugated

Product Specifications

Background

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Synonyms

HEL113; CTRCT3; Vimentin; VIM

Gene ID

7431

Swiss Prot

P08670

Cellular Locus

Cytoplasm, Extracellular matrix

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Immunogen

371-466/466

Target

Vimentin

Clonality

Polyclonal

Isotype

IgG

Conjugation

APC-Cy7

Source

KLH conjugated synthetic peptide derived from human Vimentin

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/780nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Specificity

KO-Validated

Gene ID URL

7431

Predicted Cross Reactivity

Cow, Pig, Chicken, Goat

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