Vimentin Polyclonal Antibody, APC-Cy5.5 Conjugated
Product Specifications
Background
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Synonyms
HEL113; CTRCT3; Vimentin; VIM
Gene ID
7431
Swiss Prot
P08670
Cellular Locus
Cytoplasm, Extracellular matrix
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Immunogen
371-466/466
Target
Vimentin
Clonality
Polyclonal
Isotype
IgG
Conjugation
APC-Cy5.5
Source
KLH conjugated synthetic peptide derived from human Vimentin
Applications
WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
650nm/694nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Specificity
KO-Validated
Gene ID URL
7431
Predicted Cross Reactivity
Cow, Pig, Chicken, Goat
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