HMGB1 Polyclonal Antibody, RBITC Conjugated
Product Specifications
Background
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V (D) J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS) . Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity) .
Synonyms
HMG1; HMG3; SBP-1; High mobility group protein B1; High mobility group protein 1; HMG-1; HMGB1
Gene ID
3146
Swiss Prot
P09429
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Immunogen
75-170/215
Target
HMGB1
Clonality
Polyclonal
Isotype
IgG
Conjugation
RBITC
Source
KLH conjugated synthetic peptide derived from human HMGB1
Applications
WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
570nm/595nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
3146
Predicted Cross Reactivity
Cow
Curated Selection
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