CDK4 Polyclonal Antibody, APC Conjugated
Product Specifications
Background
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G (1) /S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Synonyms
CMM3; PSK-J3; Cyclin-dependent kinase 4; Cell division protein kinase 4; CDK4
Gene ID
1019
Swiss Prot
P11802
Cellular Locus
Cytoplasm, Nucleus, Cell membrane
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Immunogen
241-303/303
Target
CDK4
Clonality
Polyclonal
Isotype
IgG
Conjugation
APC
Source
KLH conjugated synthetic peptide derived from human CDK4
Applications
WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
650nm/660nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
1019
Predicted Cross Reactivity
Cow, Pig
Curated Selection
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