Dnmt3a Polyclonal Antibody, APC-Cy7 Conjugated
Product Specifications
Background
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.
Synonyms
TBRS; DNMT3A2; M.HsaIIIA; DNA (cytosine-5) -methyltransferase 3A; Dnmt3a; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA
Gene ID
1788
Swiss Prot
Q9Y6K1
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Immunogen
26-100/912
Target
Dnmt3a
Clonality
Polyclonal
Isotype
IgG
Conjugation
APC-Cy7
Source
KLH conjugated synthetic peptide derived from human Dnmt3a
Applications
FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
650nm/780nm
Concentration
1µg/µl
Dilution
FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
1788
Predicted Cross Reactivity
Cow
Curated Selection
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