BrdU (Proliferation Marker) Polyclonal Antibody, Cy3 Conjugated
Product Specifications
Background
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis
Synonyms
Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2-deoxyuridine; 5-BrdU; proliferation Marker.
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Species independent
Target
BrdU (Proliferation Marker)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy3
Source
BrdU conjugated to KLH
Applications
IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
512,550nm/570,615nm
Concentration
1µg/µl
Dilution
IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Cross Reactivity Details
BrdU
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