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Streptavidin Polyclonal Antibody, HRP Conjugated

Product Specifications

Background

Streptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.

Synonyms

SA protein; SA V1; SA V2; Streptavidin V1; Streptavidin V2; SAV1_STRVL.

Cellular Locus

Secreted, Extracellular matrix

Host

Rabbit

Cross Reactivity

Others

Target

Streptavidin

Clonality

Polyclonal

Isotype

IgG

Conjugation

HRP

Source

Recombinant Streptavidin

Applications

ELISA, IHC-P, IHC-F

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Cross Reactivity Details

Streptavidin

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