His tag Polyclonal Antibody
Product Specifications
Background
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Synonyms
HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
Host
Rabbit
Cross Reactivity
Species independent
Target
His tag
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
Synthetic peptide: HHHHHH conjugated to KLH
Applications
WB, IHC-P
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), IHC-P (1:200-400)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Cross Reactivity Details
HHHHHH tag
Frequently Asked Questions
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