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Nestin Polyclonal Antibody, APC-Cy5.5 Conjugated

Product Specifications

Background

Required for brain and eye development (By similarity) . Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Synonyms

ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin.

Gene ID

25491

Swiss Prot

P21263

Cellular Locus

Cytoplasm

Host

Rabbit

Cross Reactivity

Mouse

Immunogen

681-750/1893

Target

Nestin

Clonality

Polyclonal

Isotype

IgG

Conjugation

APC-Cy5.5

Source

KLH conjugated synthetic peptide derived from rat Nestin

Applications

FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/694nm

Concentration

1µg/µl

Dilution

FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

25491

Predicted Cross Reactivity

Rat

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