Phospho-POLR2A (Ser5) Recombinant Antibody, Cy7 Conjugated

Product Specifications

Background

During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.

Synonyms

POLR2A; POLR2; RNA polymerase II CTD repeat YSPTSPS.

Gene ID

5430

Swiss Prot

P24928

Modification Site

Ser5

Cellular Locus

Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

POLR2A

Clonality

Recombinant

Conjugation

Cy7

Source

KLH conjugated synthetic peptide derived from human POLR2A around the phosphorylation site of S5

Applications

WB, IF, Flow-Cyt

Purification

Purified by Protein A.

Excitation Emission

743nm/767nm

Concentration

Lot dependent

Dilution

WB (1:300-5000), IF (), Flow-Cyt (1ug/Test)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

5430

Isotype

IgG

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