Phospho-POLR2A (Ser5) Recombinant Antibody
Product Specifications
Background
During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.
Synonyms
POLR2A; POLR2; RNA polymerase II CTD repeat YSPTSPS.
Gene ID
5430
Swiss Prot
P24928
Modification Site
Ser5
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
POLR2A
Clonality
Recombinant
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human POLR2A around the phosphorylation site of S5
Applications
WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF
Purification
Purified by Protein A.
Concentration
Lot dependent
Dilution
WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500), IP (1-2ug), IF (), Flow-Cyt (1ug/Test), ICC/IF (1:50-100)
Buffer
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Modification
Phosphorylation
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
5430
Isotype
IgG
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