TRIM25 Recombinant Antibody
Product Specifications
Background
Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection.
Synonyms
EFP; RNF147; TRI25; TRIM 25; Trim25; Z147; ZNF147.
Gene ID
7706
Swiss Prot
Q14258
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
TRIM25
Clonality
Recombinant
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human TRIM25
Applications
WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF
Purification
Purified by Protein A.
Concentration
Lot dependent
Dilution
WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500), IP (1-2ug), IF (), Flow-Cyt (1ug/Test), ICC/IF (1:50-100)
Buffer
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
7706
Isotype
IgG
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