Mouse anti Melanoma Associated Antigen

The mammalian members of the MAGE (melanoma-associated antigen) gene family were originally described as completely silent in normal adult tissues, with the exception of male germ cells and, for some of them, placenta. By contrast, these genes were expressed in various kinds of tumors. MAGE-like genes have also been identified in non-mammalian species, like the zebrafish or Drosophila melanogaster. Although no MAGE homologous sequences have been identified in Caenorhabditis elegans, Saccharomyces cerevisiae or Schizosaccharomyces pombe, MAGE sequences have been found in several vegetal species, including Arabidopsis thaliana. The only region of homology shared by all of the members of the family is a stretch of about 200 amino acids which has been named the MAGE conserved domain. The MAGE conserved domain is usually loCated close to the C-terminal, although it can also be found in a more central position in some proteins. The MAGE conserved domain is generally present as a single copy but it is dupliCated in some proteins. It has been proposed that the MAGE conserved domain of MAGE-D proteins might interact with p75 neurotrophin or related receptors. The function of this protein is not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. The Melanoma-associated antigen is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, leukemias and lymphomas.

NKI-C3 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Balb/c mice, immunized with a predominantly intracellular protein of Human melanoma cells.

NKI-C3 is useful for ELISA, immunofluorescence and immuohistochemistry on frozen and paraffin embedded sections. Optimal antibody dilution should be determined by titration.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

1. C. Vennegoor et al., Int. J. Cancer 35: 287-295, 1985._x000D_ 2. A.A. Palmer et al., Pathology 17: 335-339, 1985. 3.E.C. _x000D_ 3. Hagen et al., Histopathology 10: 689-700, 1986.

The antibody is shipped at ambient temperature and may be stored at +4°C; For prolonged storage prepare appropriate aliquots and store at or below -20°C; Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product