RhoA/B/C Recombinant Antibody, HRP Conjugated
Product Specifications
Background
RhoA , RhoB , RhoC is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. The three mammalian Rho proteins (A, B and C) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.
Synonyms
ARH12, ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC.
Gene ID
387
Swiss Prot
P61586, P62745, P08134
Cellular Locus
Cytoplasm, Cell membrane
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
RhoA/B/C
Clonality
Recombinant
Conjugation
HRP
Source
KLH conjugated synthetic peptide derived from human RhoA
Applications
WB, IHC-P, IHC-F
Purification
Purified by Protein A.
Concentration
Lot dependent
Dilution
WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
387
Isotype
IgG
Curated Selection
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