GLI3 Recombinant Antibody
Product Specifications
Background
Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
Synonyms
Transcriptional activator GLI3; GLI3 form of 190 kDa; GLI3 full-length protein; Transcriptional repressor GLI3R; GLI3 C-terminally truncated form; GLI3 form of 83 kDa; GLI3-190; GLI3FL; GLI3-83
Gene ID
2737
Swiss Prot
P10071
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human
Target
GLI3
Clonality
Recombinant
Conjugation
Unconjugated
Source
Recombinant protein within human Gli3 aa 1300-1600.
Applications
WB, IF (ICC)
Purification
Purified by Protein A.
Concentration
1ug/ul
Dilution
WB (1:300-5000), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
2737
Isotype
IgG
Curated Selection
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