SET Recombinant Antibody
Product Specifications
Background
Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL) -induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
Synonyms
Protein SET; HLA-DR-associated protein II; Inhibitor of granzyme A-activated DNase, PHAPII; Phosphatase 2A inhibitor I2PP2A; Template-activating factor I; IGAAD; I-2PP2A; TAF-I.
Gene ID
6418
Swiss Prot
Q01105
Cellular Locus
Cytoplasm, Nucleus, Endoplasmic reticulum
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
SET
Clonality
Recombinant
Conjugation
Unconjugated
Source
Synthetic peptide within Human SET aa 200 to the C-terminus
Applications
WB, IHC-P, IHC-F, IF, ICC/IF
Purification
Purified by Protein A.
Concentration
1ug/ul
Dilution
WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500), IF (), ICC/IF (1:50-100)
Buffer
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Modification
Unmodified
Storage Conditions
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Gene ID URL
6418
Isotype
IgG
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