Phospho-Smad2 (Ser255) Recombinant Antibody
Product Specifications
Background
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Synonyms
JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; Mad-related protein 2; SMAD family member 2; SMAD 2; Smad2
Gene ID
4087
Swiss Prot
Q15796
Modification Site
S255
Host
Rabbit
Cross Reactivity
Human, Mouse
Target
Phospho-Smad2 (Ser255)
Clonality
Recombinant
Conjugation
Unconjugated
Source
Synthetic peptide derived from human Smad2 (S255), around 220-280aa (phospho S255) .
Applications
WB, IHC-P, IHC-F, IF, ICC/IF
Purification
Purified by Protein A.
Concentration
1ug/ul
Dilution
WB (WB (1:1000) IHC (1:50-200) ), IHC-P (1:200-400), IHC-F (1:100-500), IF (), ICC/IF (1:50-100)
Buffer
0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Modification
Phosphorylation
Storage Conditions
Store at -20°C for 12 months.
Gene ID URL
4087
Isotype
IgG
Curated Selection
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