Phospho-eIF4E (Ser209) Recombinant Antibody

Product Specifications

Background

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Synonyms

CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

Gene ID

1977

Swiss Prot

P06730

Modification Site

S209

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-eIF4E (Ser209)

Clonality

Recombinant

Conjugation

Unconjugated

Source

KLH conjugated Synthesised phosphopeptide derived from human EIF4E around the phosphorylation site of Ser209: SG (p-S) TT

Applications

WB, IHC-P, IHC-F, IF, ICC/IF

Purification

Purified by Protein A.

Concentration

1ug/ul

Dilution

WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500), IF (), ICC/IF (1:50-100)

Buffer

0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Modification

Phosphorylation

Storage Conditions

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Gene ID URL

1977

Isotype

IgG

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