Phospho-LEF1 (Ser42) Polyclonal Antibody
Product Specifications
Background
Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity) . Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
Synonyms
LEF-1; TCF10; TCF7L3; TCF1ALPHA; Lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF1-alpha; LEF1
Gene ID
51176
Swiss Prot
Q9UJU2
Modification Site
Ser42
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-LEF1 (Ser42)
Clonality
Polyclonal
Conjugation
Unconjugated
Source
KLH conjugated synthetic phosphopeptide derived from human LEF1 around the phosphorylation site of Ser42
Applications
WB, IHC-P, IHC-F, IF
Purification
Purified by Protein A.
Concentration
1ug/ul
Dilution
WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500), IF ()
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Specificity
This antibody will recognize LEF1 (Ser40) in mouse and rat
Gene ID URL
51176
Isotype
IgG
Curated Selection
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