Phospho-Smad2/Smad3 (Thr8) Polyclonal Antibody, Cy3 Conjugated
Product Specifications
Background
SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF) -beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
Synonyms
Smad2 + Smad3 (phospho T8) ; p-Smad2 + Smad3 (phospho T8) ; P-Smad2 /3 (phospho T8) ; hMAD 2; hMAD 3; hSMAD2; hSMAD3; Mad related protein 2; MADH2; MADH3; MADR2; Mothers against DPP homolog 2; Mothers against DPP homolog 3; Sma and Mad related protein 2; SMA and MAD related protein 3; SMAD 2; SMAD 3; SMAD family member 2; SMAD family member 3.
Gene ID
4087
Swiss Prot
P84022
Modification Site
Thr8
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse
Target
Phospho-Smad2/Smad3 (Thr8)
Clonality
Polyclonal
Conjugation
Cy3
Source
KLH conjugated synthesised phosphopeptide derived from human Smad2/Smad3 around the phosphorylation site of Thr8
Applications
WB, FCM, IF (IHC-P), IF (ICC), IF
Purification
Purified by Protein A.
Excitation Emission
512,550nm/570,615nm
Concentration
1ug/ul
Dilution
WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (ICC) (1:50-200), IF ()
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Prediction Reactivity
Rat, Dog, Cow, Pig, Horse, Chicken
Gene ID URL
4087
Isotype
IgG
Curated Selection
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