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WT1 Polyclonal Antibody, PerCP Conjugated

Product Specifications

Background

Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.

Synonyms

GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1; Wilms tumor protein; WT1

Gene ID

7490

Swiss Prot

P19544

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Immunogen

301-400/449

Target

WT1

Clonality

Polyclonal

Conjugation

PerCP

Source

KLH conjugated synthetic peptide derived from human Wilms Tumor Protein

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

482nm/677nm

Concentration

1ug/ul

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Prediction Reactivity

Dog, Cow, Sheep, Pig, Chicken

Gene ID URL

7490

Isotype

IgG

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