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Phospho-ROCK2 (Thr249) Polyclonal Antibody, PE Conjugated

Product Specifications

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Synonyms

ROCK2phospho T249; p164 ROCK 2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; ROCK2_HUMAN.

Gene ID

9475

Modification Site

Thr249

Cellular Locus

Cytoplasm, Nucleus, Cell membrane

Host

Rabbit

Cross Reactivity

Human

Target

Phospho-ROCK2 (Thr249)

Clonality

Polyclonal

Conjugation

PE

Source

KLH conjugated synthetic phosphopeptide derived from human ROCK2 around the phosphorylation site of Thr249

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

496,564nm/578nm

Concentration

1ug/ul

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Prediction Reactivity

Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit, GuineaPig

Gene ID URL

9475

Isotype

IgG

Curated Selection

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