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Phospho-ROCK2 (Thr249) Polyclonal Antibody

Product Specifications

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Synonyms

ROCK2phospho T249; p164 ROCK 2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; ROCK2_HUMAN.

Gene ID

9475

Modification Site

Thr249

Cellular Locus

Cytoplasm, Nucleus, Cell membrane

Host

Rabbit

Cross Reactivity

Human

Target

Phospho-ROCK2 (Thr249)

Clonality

Polyclonal

Conjugation

Unconjugated

Source

KLH conjugated synthetic phosphopeptide derived from human ROCK2 around the phosphorylation site of Thr249

Applications

WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Concentration

1ug/ul

Dilution

WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Product Datasheet

https://www.biossantibodies.com/datasheets/bs-5675R

Prediction Reactivity

Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit, GuineaPig

Gene ID URL

9475

Isotype

IgG

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