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Phospho-P53 (Thr55) Polyclonal Antibody, AbBy Fluor™ 647 Conjugated

Product Specifications

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277) . [provided by RefSeq, Feb 2013].

Synonyms

P53 Thr55; P53 phospho T55; P53 phospho Thr55; p-P53 Thr55; p-P53 T55; Widespread p53; Wtp53; Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; HGNC11998; LFS1; p53; p53 Cellular Tumor Antigen; p53 Tumor Suppressor; Phosphoprotein p53; TP53; Transformation related protein 53; TRP53; Tumor protein p53; Tumour Protein p53; P53_HUMAN; TP53.

Gene ID

7157

Modification Site

Thr55

Cellular Locus

Cytoplasm, Nucleus, Cell membrane

Host

Rabbit

Cross Reactivity

Human

Target

Phospho-P53 (Thr55)

Clonality

Polyclonal

Conjugation

AbBy Fluor™ 647

Source

KLH conjugated synthetic phosphopeptide derived from human P53 around the phosphorylation site of Thr55

Applications

FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/665nm

Concentration

1ug/ul

Dilution

FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

7157

Isotype

IgG

Curated Selection

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