Phospho-PI3KCA (Tyr317) Polyclonal Antibody

Product Specifications

Background

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

Synonyms

PEPCK; PEPCK2; PEPCK-M; Phosphoenolpyruvate carboxykinase [GTP], mitochondrial; PCK2

Gene ID

5106

Swiss Prot

Q16822

Modification Site

Tyr317

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

Phospho-PI3KCA (Tyr317)

Clonality

Polyclonal

Conjugation

Unconjugated

Source

KLH conjugated synthetic phosphopeptide derived from human PI3KCA around the phosphorylation site of Tyr317

Applications

WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Concentration

1ug/ul

Dilution

WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Prediction Reactivity

Rat

Gene ID URL

5106

Isotype

IgG

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