Osteocalcin Polyclonal Antibody
Product Specifications
Background
Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite.
Synonyms
Osteocalcin, Bone Gla protein, BGP, Gamma-carboxyglutamic acid-containing protein, BGLAP
Gene ID
632
Swiss Prot
P02818
Cellular Locus
Secreted
Host
Rabbit
Cross Reactivity
Human, Mouse
Immunogen
21-100/100
Target
Osteocalcin
Clonality
Polyclonal
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human Osteocalcin
Applications
WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1ug/ul
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Prediction Reactivity
Rat
Gene ID URL
632
Isotype
IgG
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