NOX2/gp91phox Monoclonal Antibody
NOX2/gp91phox Monoclonal Antibody
Product Specifications
Product Name Alternative
CGD91-phox antibody; CYBB antibody; Cytochrome b (558) beta chain antibody; Cytochrome b-245 heavychain antibody; gp91-1 antibody; gp91-phox antibody; Heme-binding membrane glycoprotein gp91phox antibody; NADPHoxidase 2 antibody; Neutrophil cytochrome b 91 kDa polypeptide antibody; NOX2 antibody; p22 phagocyte B-cytochromeantibody; Superoxide-generating NADPH oxidase heavy chain subunit antibody
UniProt
P04839
Reactivity
Human
Immunogen
Partially Purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100)
Target
NOX2/gp91phox
Clonality
Monoclonal
Clone
54.1
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Protein G
Concentration
1mg/ml
Dilution
Optimal antibody concentration should be determined by titration, however as a guideline try; WB: Use at an assay dependent dilution. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa) .ICC/IF: Use a concentration of 1 μg/ml.IHC-P: Use a concentration of 5 μg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
SP2/0
Tested Applications
ICC, IHC-P, WB
Host or Source
Mouse
Preservative
Purified antibody containing PBS 0.1% sodium azide
Isotype
IgG1
Entrez
1536
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